Educational guide

IDENTIFYING DATA

2024_25

Subject

ADVANCED TECHNIQUES FOR ANALYSIS AND MANIPULATION OF NUCLEIC ACIDS

Code

01745002

Study programme

1745 - Máster Universitario en Investigación en Biotecnología y Biomedicina

Descriptors

Credit.

Type

Year

Period

4.5

Compulsory

First

Language

Castellano

Ingles

Prerequisites

Department

BIOLOGIA MOLECULAR

Coordinador

GARCÍA GARCÍA , PEDRO

E-mail

pgarg@unileon.es
jmferc@unileon.es
mvgarm@unileon.es
lmmatd@unileon.es
erodo@unileon.es

Lecturers

FERNÁNDEZ CAÑÓN , JOSÉ MANUEL

GARCÍA GARCÍA , PEDRO

GARCÍA MEDIAVILLA , MARÍA VICTORIA

MATEOS DELGADO , LUIS MARIANO

RODRÍGUEZ OLIVERA , ELÍAS

Web

http://

General description

The main objectives of this subject are: 1.- To gain knowledge about advanced experimental methodologies for the study of gene expression. 2.- To get a deeper knowledge about new advanced gene manipulation methodologies. 3.- To obtain knowledge about the current computer tools used in the analysis of nucleic acids. 4.- To get the capacity to design experiments in this field and to plan the work necessary to carry them out.

Tribunales de Revisión

Tribunal titular
Cargo	Departamento	Profesor
Presidente		FIERRO CASTRO , MARIA DEL CAMINO
Secretario	CIENCIAS BIOMEDICAS	SANCHEZ CAMPOS , SONIA
Vocal	BIOLOGIA MOLECULAR	SAENZ DE MIERA CARNICER , LUIS ENRIQUE

Tribunal suplente
Cargo	Departamento	Profesor
Presidente	BIOLOGIA MOLECULAR	FERRERO GARCIA , MIGUEL ANGEL
Secretario	CIENCIAS BIOMEDICAS	MERINO PELAEZ , GRACIA
Vocal	BIOLOGIA MOLECULAR	GONZALEZ CORDERO , ANA ISABEL

Competencies

Type A	Code	Competences Specific
	A18948
	A18950
	A18951
	A18953
	A18954
	A18955
	A18958
Type B	Code	Competences Transversal
	B5753
	B5754
	B5755
	B5756
	B5757
	B5758
	B5759
	B5760
	B5761
	B5763
	B5764
	B5765
	B5766
	B5767
	B5768
Type C	Code	Competences Nuclear
	C1
	C2
	C3
	C4
	C5

Learning aims

	Competences
1.- To gain knowledge about advanced experimental methodologies for the study of gene expression.	A18951 A18953 A18954 A18955	B5753 B5754 B5755 B5756 B5757 B5761 B5766 B5767 B5768	C1 C2 C3 C4 C5
2.- To get a deeper knowledge about new advanced gene manipulation methodologies.	A18950 A18951	B5753 B5754 B5755 B5756 B5757 B5766 B5767 B5768	C2 C3 C4 C5
3.- To obtain knowledge about the current computer tools used in the analysis of nucleic acids.	A18951 A18955	B5753 B5754 B5755 B5756 B5757 B5766 B5767 B5768	C1 C3 C4 C5
4.- To get the capacity to design experiments in this field and to plan the work necessary to carry them	A18948 A18953 A18958	B5753 B5754 B5755 B5756 B5757 B5758 B5759 B5760 B5761 B5763 B5764 B5765 B5766 B5767 B5768	C1 C2 C3 C4 C5

Contents

Topic	Sub-topic

1.- Advanced methods for extraction, quantification and estimation of the integrity and degree of purity of nucleic acids, especially aimed at carrying out massive sequencing experiments. 2.- Current methodologies for sequence quantification (quantitative PCR, digital PCR). 3.- Advanced genome sequencing and annotation techniques. 4.- Advanced methodologies for obtaining recombinant DNA (Synthetic Biology). 5.- Current methodologies to obtain the expression of exogenous genes. 6.- Advanced directed mutagenesis and genome editing techniques.

Planning

Methodologies :: Tests
	Class hours	Hours outside the classroom	Total hours
Laboratory practicals	30	45	75

Personal tuition	5	7.5	12.5

Lecture	10	15	25


(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies

Methodologies ::

	Description
Laboratory practicals	Practical classes (problem resolution, laboratory practices, online activities, analysis of scientific articles, annotated data recording)
Personal tuition	Personal or group interviews of students with a tutor with the aim of reviewing and clarifying possible problems that arise in the understanding or development of the teaching activity.
Lecture	Exposition of content through presentation or explanation by a teacher.

Personalized attention

Personal tuition

Lecture

Laboratory practicals

Description

For any aspect related to the subject, the student can count on the teacher's help in non-obligatory tutorials, which can be individual or in small groups.
These tutorials will be carried out by prior appointment, by phone or email.

Assessment

	Description	Qualification

Other comments and second call
Attendance at laboratory practices (attendance and active participation in all training activities). Minimum weight 20% ; Maximum weight 40% Realization, presentation and defense of works. Minimum weight 20% ; Maximum weight 40%. This section may consist of an evaluation of knowledge of the activities carried out in the laboratory practices. Delivery of a laboratory practice report. Minimum weight 20% ; Maximum weight 40% In the case of lack of attendance at the laboratory practices, and therefore lack of the related practice report, a theoretical-practical test will be carried out with a maximum value of 70% of the final grade.

Sources of information

Access to Recommended Bibliography in the Catalog ULE

Basic
	ADDGENE (2023) Plasmids 101 (4th edition). https://www.addgene.org/ BIASSONI R., RASO A. (eds.) (2014) Quantitative Real-Time PCR. Methods and Protocols. Methods in Molecular Biology (book 1160). Humana Press. BROWN, T. A. 2016. Gene cloning and DNA analysis: An introduction. Wiley-Blackwell. PATIL, N., SIVARAM, A. 2022. A complete guide to gene cloning: From basic to advanced. Springer Nature Switzerland AG. REAL GARCÍA, M., RAUSELL SEGARRA, C. LATORRE CASTILLO, A. 2017. Técnicas de Ingeniería Genética. Ed. Síntesis. SAMBROOK J., RUSSELL D.W. (2012) Molecular cloning: a laboratory manual. (4ª edición). Cold Spring Harbor Laboratory Press. SHAPIRO et al. (eds.) (2019) Ancient DNA. Methods and protocols (2ª edición). Springer. XU, J. (2014) Next-generation sequencing: Current technologies and applicattions. Caister Academic Press.
Complementary

Recommendations