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Educational guide | |||||||||||||||||||||||||||||||||||||||
IDENTIFYING DATA | 2024_25 | |||||||||||||||||||||||||||||||||||||||
Subject | ANIMAL CELL CULTURE APPLICATIONS | Code | 01745010 | |||||||||||||||||||||||||||||||||||||
Study programme |
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Descriptors | Credit. | Type | Year | Period | ||||||||||||||||||||||||||||||||||||
3 | Optional | First | Second |
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Language |
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Prerequisites | ||||||||||||||||||||||||||||||||||||||||
Department | MEDICINA,CIRUGIA Y ANAT.VETERI |
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Coordinador |
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mvvils@unileon.es mletp@unileon.es mmarm@unileon.es mmarv@unileon.es |
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Lecturers |
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Web | http:// | |||||||||||||||||||||||||||||||||||||||
General description | This subject aims to delve in a practical way with the basic techniques of cell culture and their biotechnological and health-related applications. | |||||||||||||||||||||||||||||||||||||||
Tribunales de Revisión |
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Competencies |
Type A | Code | Competences Specific |
A18948 | ||
A18953 | ||
A18956 | ||
A18957 | ||
A18958 | ||
Type B | Code | Competences Transversal |
B5753 | ||
B5754 | ||
B5755 | ||
B5757 | ||
B5758 | ||
B5760 | ||
B5762 | ||
B5763 | ||
B5764 | ||
B5765 | ||
B5766 | ||
B5767 | ||
B5768 | ||
Type C | Code | Competences Nuclear |
C1 | ||
C2 | ||
C4 | ||
C5 |
Learning aims |
Competences | |||
A18948 |
B5753 B5754 B5755 B5757 B5764 B5765 B5766 B5767 B5768 |
C1 C2 C4 C5 |
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A18948 A18958 |
B5755 B5757 B5764 |
C1 C2 C4 C5 |
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A18948 A18953 A18956 A18957 A18958 |
B5753 B5754 B5755 B5757 B5758 B5760 B5762 B5763 B5764 B5765 B5766 B5767 B5768 |
C1 C2 C4 C5 |
Contents |
Topic | Sub-topic |
PRIMARY CELL CULTURES | • Establishment of primary cell cultures for use in biomedical applications. • Cellular models of differentiation. |
GENETIC MODIFICATION | • Animal cell transfection and transduction methods. Generation of stable cell lines that express genes of interest. |
IN VITRO INFECTION MODELS | • In vitro infection tests of biomedical interest. |
Planning |
Methodologies :: Tests | |||||||||
Class hours | Hours outside the classroom | Total hours | |||||||
Laboratory practicals | 20 | 30 | 50 | ||||||
Personal tuition | 1 | 1.5 | 2.5 | ||||||
Portfolios/Learning folder | 1 | 1.5 | 2.5 | ||||||
Lecture | 8 | 12 | 20 | ||||||
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies |
Description | |
Laboratory practicals | In-person practical sessions. Experimental tests, problems or computer simulations. Students will carry out or follow an experimental protocol following good laboratory practices, the guide to good practices for handling experimental animals and/or genetically manipulated microorganisms. In these sessions, the results obtained will be discussed by the students and the teacher, analysing their importance and interest. |
Personal tuition | Specialized face-to-face online tutoring through educational support platforms. |
Portfolios/Learning folder | Self-training activities: -Analysis of relevant scientific articles on current topics related to the subject. Preparation of written reports, oral presentations or seminars on experimental or bibliographic work. - Carrying out autonomous tasks online or in a computer room. |
Lecture | Face-to-face theoretical sessions. Presentation or discussion of the contents of the subject. |
Personalized attention |
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Assessment |
Description | Qualification | ||
Others | Attendance at laboratory practices (attendance and active participation in all training activities). Delivery of practice report. |
60 40% |
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Other comments and second call | |||
Sources of information |
Access to Recommended Bibliography in the Catalog ULE |
Basic | |
Freshney RI, Culture of Animal Cells. A Manual of BasicTechnique, Fourth Edition Ranjna C. Dutta, Aroop K. Dutta. 3D Cell Culture: Fundamentals and Applications in Tissue Engineering and Regenerative Medicine. Cell Culture and Its Application. Ronald T. Acton, J. Daniel Lynn. |
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Complementary | |
All the material necessary to follow the subject will be posted on Moodle. |
Recommendations |
Subjects that it is recommended to have taken before | ||
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