|Subject||GENE EXPRESSION REGULATION IN EUKARYOTES||Code||00208055|
|General description||Characteristics of the gene expression in eukaryotes. Genome size and complexity. Chromatin organization. Transcription. Transcription factors and elements. Transcriptional regulation. Pos-transcriptional regulation. Development. Complex regulation case studies.|
|Tribunales de Revisión||
|To have a comprehensive view on the control of gene expression, being able to relate different levels of knowledge: - The different components that intervene in eukaryotic gene regulation and the way they interact with each other. - The different stages in which gene expression can be regulated. - Signal transmission and their effect on gene expression. - Complex examples on gene regulation.||A14023
|Being able to apply the adquired knowledge in biotechnology.||A14091
|Being able to make an oral presentation, using slides, to a non-professional audience.||A14022
|Lectures on Regulation||Unit 1.- Size and complexity of the genomes.
Unit 2.- Transcription. Promoters and general transcription machinery.
Unit 3.- Transcription factors. Activators and repressors.
Unit 4.- Processing of RNAs.
Unit 5.- Alternative splicing regulation.
Unit 6.- Regulation of non-coding RNAs.
Unit 7.- Chromatin and effect of the state of chromatin on regulation.
Unit 8.- Epigenetics.
Unit 9.- Mono-allelic expression.
Unit 10.- Regulation by copy number variation (CNV).
|Short seminars||Unit 11.- Examples in animals and plants (seminars by students).|
|Practical work||Observation of the effects of differential gene expression on the development of nematodes.|
|Methodologies :: Tests|
|Class hours||Hours outside the classroom||Total hours|
|Presentations / expositions||4||6||10|
|(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.|
|Laboratory practicals||Practical application of theoretical knowledge|
|Presentations / expositions||Students will make a presentation on a topic of their choice. This presentation can be in either English or Spanish. Alternatively, if the professor aprove it, students can write an paper on the topic.|
|Lecture||Exposure of the fundamental data of the subject. Presentation of practical cases and applications.|
|Mixed tests||The level of knowledge, the ability to relate different concepts and the clarity and precision in the presentation of ideas will be evaluated. The presentation of a work on a topic picked by the students will also be evaluated. Works can be presented in either English or Spanish. Alternatively, students can write a paper on the chosen topic.
Attendance and participation in classes will be evaluated.
Instrument handling, attitude in the laboratory and interpretation of results will be evaluated.
|Other comments and second call|
Presentation by the student (or in the final exam, if applicable): 60%. In case of a presentation, percentages will be distributed as follows, 20% quality of the work, 30% knowledge on the topic, and 10% exposure by the student.
Continuous evaluation: 25%, which includes participation in class and quizes.
Laboratory work: 15%
During theperformance of exams and evaluation tests, the use and possession of any typeof materials, means and resources that serve as a source of information isprohibited, except those that may be specifically authorized by the professorin each of the tests. Failure to comply carries the grade of fail.
|Sources of information|
|Access to Recommended Bibliography in the Catalog ULE|
General books on Molecular Genetics cover most of the topics.
Carlberg and Molnar. 2016. Mechanisms of Gene Regulation. Springer.
David S. Latchman. 2004. Eukaryotic transcription factors. Fourth Edition. Elsevier Academic Press.
|Subjects that it is recommended to have taken before|