Educational guide
IDENTIFYING DATA 2023_24
Subject GENE EXPRESSION REGULATION IN EUKARYOTES Code 00208055
Study programme
0208 - GRADO EN BIOTECNOLOGÍA
Descriptors Credit. Type Year Period
3 Optional Fourth Second
Language
Castellano
Prerequisites
Department BIOLOGIA MOLECULAR
Coordinador
FIERRO CASTRO , MARIA DEL CAMINO
E-mail mcfiec@unileon.es
jgutg@unileon.es
Lecturers
FIERRO CASTRO , MARIA DEL CAMINO
GUTIERREZ GONZALEZ , JUAN JOSE
Web http://
General description Characteristics of the gene expression in eukaryotes. Genome size and complexity. Chromatin organization. Transcription. Transcription factors and elements. Transcriptional regulation. Pos-transcriptional regulation. Development. Complex regulation case studies.
Tribunales de Revisión
Tribunal titular
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR GARCIA GARCIA , PEDRO
Secretario BIOLOGIA MOLECULAR SAENZ DE MIERA CARNICER , LUIS ENRIQUE
Vocal BIOLOGIA MOLECULAR FERRERO GARCIA , MIGUEL ANGEL
Tribunal suplente
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR RODRIGUEZ APARICIO , LEANDRO B.
Secretario BIOLOGIA MOLECULAR MARTINEZ BLANCO , HONORINA
Vocal BIOLOGIA MOLECULAR POLANCO DE LA PUENTE , CARLOS

Competencias
Code  
A14022
A14023
A14091
B3766
B3767
B3772
B3787
C3 CMECES3 That students have the ability to gather and interpret relevant data (normally within their area of study) to make judgments that include reflection on relevant issues of a social, scientific or ethical nature.
C4 CMECES4 That students can transmit information, ideas, problems and solutions to both a specialised and non-specialised audience

Learning aims
Competences
To have a comprehensive view on the control of gene expression, being able to relate different levels of knowledge: - The different components that intervene in eukaryotic gene regulation and the way they interact with each other. - The different stages in which gene expression can be regulated. - Signal transmission and their effect on gene expression. - Complex examples on gene regulation. A14023
 B3772
B3787
 C3
Being able to apply the adquired knowledge in biotechnology. A14091
 B3766
 C3
Being able to make an oral presentation, using slides, to a non-professional audience. A14022
 B3767
 C4

Contents
Topic Sub-topic
Lectures on Regulation Unit 1.- Size and complexity of the genomes.
Unit 2.- Transcription. Promoters and general transcription machinery.
Unit 3.- Transcription factors. Activators and repressors.
Unit 4.- Processing of RNAs.
Unit 5.- Alternative splicing regulation.
Unit 6.- Regulation of non-coding RNAs.
Unit 7.- Chromatin and effect of the state of chromatin on regulation.
Unit 8.- Epigenetics.
Unit 9.- Mono-allelic expression.
Unit 10.- Regulation by copy number variation (CNV).
Short seminars Unit 11.- Examples in animals and plants (seminars by students).
Practical work Observation of the effects of differential gene expression on the development of nematodes.

Planning
Methodologies  ::  Tests
  Class hours Hours outside the classroom Total hours
Laboratory practicals 10 15 25
 
Presentations / expositions 4 6 10
 
Lecture 14 21 35
 
Mixed tests 2 3 5
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies   ::  
  Description
Laboratory practicals Practical application of theoretical knowledge
Presentations / expositions Students will make a presentation on a topic of their choice. This presentation can be in either English or Spanish. Alternatively, if the professor aprove it, students can write an paper on the topic.
Lecture Exposure of the fundamental data of the subject. Presentation of practical cases and applications.

Personalized attention
 
Lecture
Laboratory practicals
Presentations / expositions
Description
Tutoring with students upon request for an interview by e-mail. They are intended to solve individual questions that may arise during the course.

Assessment
  Description Qualification
Lecture Attendance and participation in classes will be considered. .
Laboratory practicals The handling of instruments, the attitude in the laboratory and the interpretation of results will be evaluated. .
Presentations / expositions The presentation of a final work by the students will be evaluated. Throughout the course, other tasks may be proposed, such as discussions on relevant scientific articles. .
Mixed tests The level of knowledge, the ability to relate different concepts and the clarity and precision in the presentation of ideas will be evaluated. The presentation of a work on a topic picked by the students will also be evaluated. Works can be presented in either English or Spanish. Alternatively, students can write a paper on the chosen topic. .
 
Other comments and second call

+ Presentation by the student (or final exam if applicable): 60%. In case of presentation, it will be distributed as follows:

20% quality of work. The work is adjusted to the chosen topic. Quality of the slides (quality and quantity of theoretical and graphic material).

20% theoretical knowledge shown by the student in the presentation.

10% exhibition of the work by the student. Ability to answer the questions that are proposed.

10% participation in peer presentations. Questions and participation in debates that arose.

+ Continuous evaluation: 25%, which includes participation in class and tests or assignments done during the course.

+ Lab work: 15%

During the exams and evaluation tests, the use and possession of any type of materials, media and resources that serve as a source of information is prohibited, except those that the teacher may specifically authorize in each of the tests. Failure to comply will result in a fail rating.

In the second call, for the final grade the following will be taken into account: a final exam on the contents of the course (60%), results of the continuous evaluation (25%) and the performance of laboratory practices (15%)

Sources of information
Access to Recommended Bibliography in the Catalog ULE

Basic

General books on Molecular Genetics cover most of the topics.

Specific bibliography:

Carlberg and Molnar. 2016. Mechanisms of Gene Regulation. Springer.
Complementary

http://www.sciencemag.org/content/vol319/issue5871/index.dtl

Anna Petherick, Nature, 454:1043-1045, 2008" "

Recommendations


Subjects that it is recommended to have taken before
Genetics / 00208013
MOLECULAR GENETICS / 00208018
BIOINFORMATICS / 00208021
Genomics / 00208024