Guia docente
DATOS IDENTIFICATIVOS 2016_17
Asignatura CULTIVO E IDENTIFICACIÓN DE MICROORGANISMOS Código 00212009
Enseñanza
M.U.INVEST.EN BIOLOGIA FUNDA.Y BIOMEDICINA
Descriptores Cr.totales Tipo Curso Semestre
5 Optativa Primer Anual
Idioma
Castellano
Ingles
Prerrequisitos
Departamento BIOLOGIA MOLECULAR
Responsable
CASQUEIRO BLANCO , FRANCISCO JAVIER
Correo-e fjcasb@unileon.es
sgutm@unileon.es
jjrubc@unileon.es
Profesores/as
CASQUEIRO BLANCO , FRANCISCO JAVIER
GUTIÉRREZ MARTÍN , SANTIAGO
RUBIO COQUE , JUAN JOSÉ
Web http://www.unileon.es/estudiantes/estudiantes-master/oferta-titulaciones/mu-metodologia-investigacion-biologia-biomedicina/plan-estudios?id=0212009&curso=2013
Descripción general Profundizar en la identificación molecular de bacterias, levaduras, hongos
Tribunales de Revisión
Tribunal titular
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR GIL SANTOS , JOSE ANTONIO
Secretario BIOLOGIA MOLECULAR CASTRO GONZALEZ , JOSE MARIA
Vocal BIOLOGIA MOLECULAR MATEOS DELGADO , LUIS MARIANO
Tribunal suplente
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR APARICIO FERNANDEZ , JESUS
Secretario BIOLOGIA MOLECULAR POLANCO DE LA PUENTE , CARLOS
Vocal BIOLOGIA MOLECULAR RODRIGUEZ APARICIO , LEANDRO B.

Competencias
Tipo A Código Competencias Específicas
  A13049 212CE4 Capacidad para utilizar recursos informáticos relacionados con el manejo de bases de datos, tanto bibliográficas como relativas a las moléculas biológicas y a las particularidades de sus métodos de análisis.
  A13050 212CE5 Capacidad de utilización de los métodos matemáticos y estadísticos adecuados para para analizar los datos obtenidos en estudios biológicos o médicos, así como su edición y presentación en documentos científicos.
  A13054 212CE9 Capacidad para utilizar apropiadamente el equipamiento necesario para obtener cultivos en esterilidad, eliminar los residuos de forma apropiada, especialmente los microrganismos modificados genéticamente y descontaminar el equipamiento si fuera necesario.
Tipo B Código Competencias Generales y Transversales
  B2890 212CG1 Proporcionar una formación teórico-práctica de los fundamentos metodológicos de investigación básica y aplicada para el desarrollo de actividades de I+D+I en Biología Fundamental, Biomedicina y Veterinaria orientada a seguir estudios posteriores y al ejercicio profesional.
  B2892 212CG3 Aprender a buscar información, a seleccionarla,a reunir e interpretar datos relevantes sobre diferentes temas.
  B2893 212CG4 Conseguir habilidades en el planteamiento de experimentos, planificación del trabajo, uso racional de los medios y de los recursos, recogida y análisis de los datos, su registro fiel, análisis, presentación y defensa de los mismos.
  B2894 212CT1 Expresión oral y escrita
  B2895 212CT10 Trabajo en equipo
  B2896 212CT11 Adaptación a nuevas situaciones, iniciativa y espiritu emprendedor
  B2897 212CT12 Pensamiento crítitco
  B2898 212CT13 Mantener un compromiso ético
  B2899 212CT2 Solucion de problemas
  B2900 212CT3 Utilizar Internet como medio de comunicación y como fuente de información
  B2901 212CT4 Habilidad de comunicación
  B2902 212CT5 Organizar y planificar el trabajo
  B2903 212CT6 Tomar decisiones
  B2904 212CT7 Liderazgo
  B2905 212CT8 Capacidad de autoevaluación
  B2906 212CT9 Creatividad
Tipo C Código Competencias Básicas
  C1 Que los estudiantes sepan aplicar los conocimientos adquiridos y su capacidad de resolución de problemas en entornos nuevos o poco conocidos dentro de contextos más amplios (o multidisciplinares) relacionados con su área de estudio.
  C2 Que los estudiantes sean capaces de integrar conocimientos y enfrentarse a la complejidad de formular juicios a partir de una información que, siendo incompleta o limitada, incluya reflexiones sobre las responsabilidades sociales y éticas vinculadas a la aplicación de sus conocimientos y juicios.
  C3 Que los estudiantes sepan comunicar sus conclusiones (y los conocimientos y razones últimas que las sustentan) a públicos especializados y no especializados de un modo claro y sin ambigüedades.
  C4 Que los estudiantes posean las habilidades de aprendizaje que les permitan continuar estudiando de un modo que habrá de ser en gran medida autodirigido o autónomo.

Resultados de aprendizaje
Resultados Competencias
Identificación Molecular de Bacterias Identificación Molecular de Levaduras Identificación Molecular de Hongos Filamentosos Métodos de aislamiento de microorganismos Normas de Seguridad en el Laboratorio de Microbiología Manejo de bases de datos de DNA Elaboración de árboles filogenéticos Aislamiento y selección de levaduras de interés enológico. Discriminación entre Saccharomyces cerevisiae y otras especies: análisis RFLP-5.8S-ITS Identificación molecular de levaduras: secuanción de los dominios D1 y D2 del ADNr 26S. Tipaje molecular de S. cerevisiase: RFLP de ADN mitocondrial. A13049
A13050
A13054
 B2890
B2892
B2893
B2894
B2895
B2896
B2897
B2898
B2899
B2900
B2901
B2902
B2903
B2904
B2905
B2906
 C1
C2
C3
C4

Contenidos
Bloque Tema
-Bioseguridad en el laboratorio. -El RNAr 16S en la identificación bacteriana. -Métodos de aislamiento de hongos filamentosos. -Identificación de hongos filamentosos en base a los ITS1 e ITS2. -Elaboración de árboles filogenéticos. -Análisis de características fenotípicas que se correlacionen con las distancias observadas en los árboles filogenéticos. -Aislamiento y selección de levaduras de interés enológico. -Discriminación entre Saccharomyces cerevisiae y otras especies: análisis RFLP-5.8S-ITS. -Identificación molecular de levaduras: secuanción de los dominios D1 y D2 del ADNr 26S. -Tipaje molecular de S. cerevisiase: RFLP de ADN mitocondrial.

Planificación
Metodologías  ::  Pruebas
  Horas en clase Horas fuera de clase Horas totales
Prácticas en laboratorios 69 0 69
 
Trabajos 41 0 41
 
Sesión Magistral 15 0 15
 
 
(*)Los datos que aparecen en la tabla de planificación són de carácter orientativo, considerando la heterogeneidad de los alumnos

Metodologías
Metodologías   ::  
  descripción
Prácticas en laboratorios Ensayos experimentales, problemas o simulaciones informáticas. Los estudiantes realizarán o seguirán un protocolo experimental siguiendo las buenas prácticas de laboratorio, la guía de buenas prácticas de manipulación de animales de experimentación y/o microorganismos manipulados genéticamente. En dichas sesiones se discutirán, por parte de los estudiantes y el profesor, los resultados obtenidos, analizando su importancia e interés.
Trabajos Análisis de artículos científicos relevantes sobre temas actuales relacionados con la materia. - Preparación de memorias escritas, exposiciones orales o seminarios sobre trabajo experimental o bibliográfico. - Realización de tareas autónomas online o en aula informática.
Sesión Magistral Exposición o discusión de los contenidos de la materia.

Tutorías
 
Sesión Magistral
Prácticas en laboratorios
Trabajos
descripción
Tutorías especializadas presenciales y/o online mediante plataformas educativas de apoyo.

Evaluación
  descripción calificación
Sesión Magistral
Prácticas en laboratorios
Trabajos
 
Otros comentarios y segunda convocatoria
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priority=&quot;63&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Shading 1 Accent 4&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;64&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Shading 2 Accent 4&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;65&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium List 1 Accent 4&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;66&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium List 2 Accent 4&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;67&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 1 Accent 4&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;68&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 2 Accent 4&quot;/> <w:lsdexception locked=&quot;false&quot; 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priority=&quot;67&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 1 Accent 5&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;68&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 2 Accent 5&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;69&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 3 Accent 5&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;70&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Dark List Accent 5&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;71&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Colorful Shading Accent 5&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;72&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Colorful List Accent 5&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;73&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Colorful Grid Accent 5&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;60&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Light Shading Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;61&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Light List Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;62&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Light Grid Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;63&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Shading 1 Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;64&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Shading 2 Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;65&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium List 1 Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;66&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium List 2 Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;67&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 1 Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;68&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 2 Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;69&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Medium Grid 3 Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; priority=&quot;70&quot; semihidden=&quot;false&quot; unhidewhenused=&quot;false&quot; name=&quot;Dark List Accent 6&quot;/> <w:lsdexception locked=&quot;false&quot; 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Acceso a la Lista de lecturas de la asignatura

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Los alumnos deberán buscar las fuentes de información dependiendo del tipo de trabajo a realizar utilizando los accesos informáticos proporcionados por el profesor. El portal de inicio será la página http://www.ncbi.nlm.nih.gov/pubmed/. El alumno dispondrá de fuentes básicas de información en la plataforma educativa.
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