Educational guide | ||||||||||||||||||||||||||||||||||||||||
IDENTIFYING DATA | 2023_24 | |||||||||||||||||||||||||||||||||||||||
Subject | ADVANCED TECHNIQUES FOR ANALYSIS AND MANIPULATION OF NUCLEIC ACIDS | Code | 01745002 | |||||||||||||||||||||||||||||||||||||
Study programme |
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Descriptors | Credit. | Type | Year | Period | ||||||||||||||||||||||||||||||||||||
4.5 | Compulsory | First | First |
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Language |
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Prerequisites | ||||||||||||||||||||||||||||||||||||||||
Department | BIOLOGIA MOLECULAR |
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Coordinador |
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pgarg@unileon.es jmferc@unileon.es mvgarm@unileon.es lmmatd@unileon.es erodo@unileon.es |
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Lecturers |
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Web | http:// | |||||||||||||||||||||||||||||||||||||||
General description | The main objectives of this subject are: 1.- To gain knowledge about advanced experimental methodologies for the study of gene expression. 2.- To get a deeper knowledge about new advanced gene manipulation methodologies. 3.- To obtain knowledge about the current computer tools used in the analysis of nucleic acids. 4.- To get the capacity to design experiments in this field and to plan the work necessary to carry them out. | |||||||||||||||||||||||||||||||||||||||
Tribunales de Revisión |
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Competencies |
Type A | Code | Competences Specific |
A18948 | ||
A18950 | ||
A18951 | ||
A18953 | ||
A18954 | ||
A18955 | ||
A18958 | ||
Type B | Code | Competences Transversal |
B5753 | ||
B5754 | ||
B5755 | ||
B5756 | ||
B5757 | ||
B5758 | ||
B5759 | ||
B5760 | ||
B5761 | ||
B5763 | ||
B5764 | ||
B5765 | ||
B5766 | ||
B5767 | ||
B5768 | ||
Type C | Code | Competences Nuclear |
C1 | ||
C2 | ||
C3 | ||
C4 | ||
C5 |
Learning aims |
Competences | |||
A18951 A18953 A18954 A18955 |
B5753 B5754 B5755 B5756 B5757 B5761 B5766 B5767 B5768 |
C1 C2 C3 C4 C5 |
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A18950 A18951 |
B5753 B5754 B5755 B5756 B5757 B5766 B5767 B5768 |
C2 C3 C4 C5 |
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A18951 A18955 |
B5753 B5754 B5755 B5756 B5757 B5766 B5767 B5768 |
C1 C3 C4 C5 |
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A18948 A18953 A18958 |
B5753 B5754 B5755 B5756 B5757 B5758 B5759 B5760 B5761 B5763 B5764 B5765 B5766 B5767 B5768 |
C1 C2 C3 C4 C5 |
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1.- To gain knowledge about advanced experimental methodologies for the study of gene expression. 2.- To get a deeper knowledge about new advanced gene manipulation methodologies. 3.- To obtain knowledge about the current computer tools used in the analysis of nucleic acids. 4.- To get the capacity to design experiments in this field and to plan the work necessary to carry them |
Contents |
Topic | Sub-topic |
1.- Advanced methods for extraction, quantification and estimation of the integrity and degree of purity of nucleic acids, especially aimed at carrying out massive sequencing experiments. • 2.- Current methodologies for sequence quantification (quantitative PCR, digital PCR). • 3.- Advanced genome sequencing and annotation techniques. • 4.- Advanced methodologies for obtaining recombinant DNA (Synthetic Biology). • 5.- Current methodologies to obtain the expression of exogenous genes. • 6.- Advanced directed mutagenesis and genome editing techniques. |
Planning |
Methodologies :: Tests | |||||||||
Class hours | Hours outside the classroom | Total hours | |||||||
Laboratory practicals | 30 | 45 | 75 | ||||||
Personal tuition | 5 | 7.5 | 12.5 | ||||||
Lecture | 10 | 15 | 25 | ||||||
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies |
Description | |
Laboratory practicals | Practical classes (problem resolution, laboratory practices, online activities, analysis of scientific articles, annotated data recording) |
Personal tuition | Personal or group interviews of students with a tutor with the aim of reviewing and clarifying possible problems that arise in the understanding or development of the teaching activity. |
Lecture | Exposition of content through presentation or explanation by a teacher. |
Personalized attention |
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Assessment |
Description | Qualification | ||
Others | Attendance at laboratory practices (attendance and active participation in all training activities). Realization, presentation and defense of works. Delivery of a laboratory practice report. |
20-40 20-40 20-40 |
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Other comments and second call | |||
Sources of information |
Access to Recommended Bibliography in the Catalog ULE |
Basic | |
-BIASSONI R., RASO A. (eds.) (2014) Quantitative Real-Time PCR. Methods and Protocols. Methods in Molecular Biology (book 1160). Humana Press. -BROWN, T. A. 2016. Gene cloning and DNA analysis: An introduction. WileyBlackwell. -PERERA J., TORMO A., GARCÍA J.L. (2002) Ingeniería genética. Ed. Síntesis. -PRIMROSE S.B., TWYMAN R.M. (2006) Principles of Gene Manipulation and Genomics (7th edition). Blackwell Publishing. -REAL GARCÍA, M., RAUSELL SEGARRA, C. LATORRE CASTILLO, A. 2017. Técnicas de Ingeniería Genética. Ed. Síntesis. -SAMBROOK J., RUSSELL D.W. (2012) Molecular cloning: a laboratory manual. (4ª edición). Cold Spring Harbor Laboratory Press. -SHAPIRO et al. (eds.) (2019) Ancient DNA. Methods and protocols (2ª edición). Springer. -XU, J. (2014) Next-generation sequencing: Current technologies and applicattions. Caister Academic Press. |
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Complementary | |
Recommendations |