Educational guide
IDENTIFYING DATA 2023_24
Subject PROTEIN STUDY METHODS Code 01745003
Study programme
1745 - Máster Universitario en Investigación en Biotecnología y Biomedicina
Descriptors Credit. Type Year Period
4.5 Compulsory First First
Language
Castellano
Ingles
Prerequisites
Department CIENCIAS BIOMEDICAS
Coordinador
GARCÍA MEDIAVILLA , MARÍA VICTORIA
E-mail mvgarm@unileon.es
mjcueg@unileon.es

aferl@unileon.es
maferg@unileon.es
jlmaug@unileon.es
ssanc@unileon.es
cbarm@unileon.es
bestg@unileon.es
bmarf@unileon.es
Lecturers
CUEVAS GONZÁLEZ , MARÍA JOSE
FERNÁNDEZ ALONSO , ROSALIA
FERNÁNDEZ LÓPEZ , ARSENIO
FERRERO GARCÍA , MIGUEL ÁNGEL
GARCÍA MEDIAVILLA , MARÍA VICTORIA
MAURIZ GUTIÉRREZ , JOSE LUIS
SÁNCHEZ CAMPOS , SONIA
BARREIRO MENDEZ , CARLOS
ESTEBANEZ GONZALEZ , BRISAMAR
MARTIN FERNANDEZ , BEATRIZ
Web http://www.unileon.es/estudiantes/estudiantes-master/oferta-titulaciones/mu-metodologia-investigacion-biologia-biomedicina/plan-estudios?id=0212006&curso=2013
General description Methods of study of protein.
Tribunales de Revisión
Tribunal titular
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR GARCIA GARCIA , PEDRO
Secretario BIOLOGIA MOLECULAR MARIN VIEIRA , MARIA DEL CARMEN
Vocal CIENCIAS BIOMEDICAS MERINO PELAEZ , GRACIA
Tribunal suplente
Cargo Departamento Profesor
Presidente CIENCIAS BIOMEDICAS GONZALEZ GALLEGO , JAVIER
Secretario BIOLOGIA MOLECULAR FERNANDEZ CAÑON , JOSE MANUEL
Vocal MEDICINA,CIRUGIA Y ANAT.VETERI VILLAR SUAREZ , MARIA VEGA

Competencies
Type A Code Competences Specific
  A18948
  A18953
  A18955
Type B Code Competences Transversal
  B5753
  B5754
  B5755
  B5756
  B5757
  B5758
  B5759
  B5760
  B5761
  B5762
  B5763
  B5764
  B5765
  B5766
  B5767
  B5768
Type C Code Competences Nuclear
  C1
  C2
  C3
  C4
  C5

Learning aims
Competences
A18948
A18953
A18955
 B5753
B5754
B5755
B5756
B5757
B5758
B5760
B5761
B5766
B5767
B5768
 C1
C2
C3
C4
A18948
A18953
A18955
 B5753
B5754
B5755
B5756
B5757
B5766
B5767
B5768
 C1
C2
C3
C4
A18948
A18953
A18955
 B5753
B5754
B5755
B5756
B5757
B5758
B5759
B5760
B5761
B5762
B5763
B5764
B5765
B5766
B5767
B5768
 C1
C2
C3
C4
C5
A18948
A18953
A18955
 B5753
B5754
B5755
B5756
B5757
B5758
B5759
B5760
B5761
B5762
B5763
B5764
B5765
B5766
B5767
B5768
 C1
C2
C3
C4
A18948
A18953
A18955
 B5754
B5755
B5756
B5757
B5758
B5759
B5760
B5762
B5764
B5766
B5767
B5768
 C1
C2
C3
C4
C5

Contents
Topic Sub-topic
1.-UPDATE ON PROTEIN EXTRACTION, QUANTIFICATION AND PURIFICATION METHODS. APPLICATION IN RESEARCH: -Obtaining total proteins from tissue samples/cell cultures
-Quantification of total proteins in the samples
-Protein purification
-Identification of proteins using antibodies:
Fundamentals and applications of Western blot, dot blot and ELISA techniques.
Image analysis techniques for protein quantification: Fundamentals and applications of flow cytometry and fluorescence microscopy techniques. Fundamentals and applications of immunohistochemistry techniques.
2.-UPDATE IN THE IDENTIFICATION, QUANTIFICATION AND LOCATION OF PROTEINS. APPLICATION IN RESEARCH: -Fundamentals of enzyme kinetics. Cellular localization of enzymatic activities using histoenzymatic techniques - Analysis and interpretation of the results.
-Two-dimensional protein electrophoresis.
-Protein analysis by mass spectrometry (MALDI-TOF, peptide fingerprinting, analysis of post-translational modifications, etc.).
-Protein labeling.

Planning
Methodologies  ::  Tests
  Class hours Hours outside the classroom Total hours
Laboratory practicals 30 45 75
 
Personal tuition 5 7.5 12.5
 
Lecture 10 15 25
 
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies   ::  
  Description
Laboratory practicals Experimental tests, problems or computer simulations. Students will carry out or follow an experimental protocol following good laboratory practices, the guide to good practices for handling experimental animals and/or genetically manipulated microorganisms. In these sessions, the results obtained will be discussed by the students and the teacher, analysing their importance and interest. In addition to laboratory practices, the student will carry out self-training activities: Analysis of relevant scientific articles on current topics related to the subject. Preparation of written reports, oral presentations or seminars on experimental or bibliographic work. Carrying out autonomous tasks online or in a computer room.
Personal tuition Specialized online tutoring through supportive and/or in-person educational platforms.
Lecture Presentation or discussion of the contents of the subject.

Personalized attention
 
Lecture
Laboratory practicals
Personal tuition
Description
Specialized online tutoring through educational platforms and/or in-person.

Assessment
  Description Qualification
Others The following aspects will be evaluated:
-Attendance at laboratory practices (attendance and active participation in all training activities). Your grade will represent between 40 and 50% of the final grade.
-Performance, presentation and/or defense of works. Your grade will represent between 15 and 25% of the final grade.
-Delivery of practice report. Your grade will represent between 20 and 25% of the final grade.
-Problem and case resolution. Content questionnaires. Your grade will represent between 15 and 20% of the final grade.
 
Other comments and second call

Continuous assessment. The qualification system will comply with the provisions of RD 1125/2003.

As provided, the adapted teaching criteria that reflect the ULE Action Plan in force will be used, depending on the health situation at the time.

The submitted works may be reviewed with an anti-plagiarism computer program. If plagiarism is detected, the work will be graded as failed.

During the evaluation tests, it will not be possible to use printed materials or electronic resources (calculators, tablets, phones, computers, etc.), except for those tests that, under the express direction of the teacher, require the use of any of these resources. If any irregularity occurs during the corresponding exam or evaluation test, the exam will be immediately withdrawn, the student will be expelled and graded as a fail. In any case, the provisions of the internal regulations of the ULe included in the document "Guidelines for action in cases of plagiarism, copying or fraud in exams or evaluation tests" will be followed (Approved Permanent Commission of the Governing Council 01/29 /2015).

Sources of information
Access to Recommended Bibliography in the Catalog ULE

Basic

Katherine M. McKinnon. Flow Cytometry: An Overview. Curr Protoc Immunol. 120: 5.1.1-

5.1.11. (2018) doi: 10.1002/cpim.40

Peter O. Krutzik, Jonathan M. Irish, Garry P. Nolan, and Omar D. Perezb.Analysis of protein phosphorylation and cellular signaling events by flow cytometry: techniques and clinical applications. Clinical Immunology 110 (2004) 206– 221.

Wolfgang Hül, Jose Iturraspe, Guillermo A. Martinez, Charles E. Hutcheson, Cheryl G. Roberts, Diann D. Fisk, Michelle W. Sugrue, John R. Wingard, and Raul C. Braylan.

Measurement of Absolute Concentration and Viability Of CD341 Cells in Cord Blood and Cord Blood Products Using Fluorescent Beads and Cyanine Nucleic Acid Dyes. Cytometry (Communications in Clinical Cytometry) 34:121–127 (1998).

http://www.westernblotting.org/http://www.molecularstation.com/protein/western-blot/

Sambrook J, Fritsch EF and Maniatis T. Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press. 1989. Chapters 17 and 18.

Hockfields S, Carlson S, Evans C, Levitt P, Pintar J, Silberstein L. Selected methods for antibodies and nucleic acid probes. Cold Spring Harbor Laboratory Press. 1989. Capitulos 2 y 3.

Cuello AC. Immunohistochemistry II. IBRO Handbook Series: Methods in the Neurosciencies. Powers, J. L., Kiesman, N. E., Tran, C. M., Brown, J. H., & Bevilacqua, V. L. H. (2007).

Lactate dehydrogenase kinetics and inhibition using a microplate reader. Biochemistry and Molecular Biology Education: A Bimonthly Publication of the International Union of Biochemistry and Molecular Biology, 35(4), 287-292. https://doi.org/10.1002/bmb.74
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