Educational guide
IDENTIFYING DATA 2023_24
Subject MEDICAL MICROBIOLOGY TESTS Code 01745004
Study programme
1745 - Máster Universitario en Investigación en Biotecnología y Biomedicina
Descriptors Credit. Type Year Period
3 Compulsory First First
Language
Castellano
Prerequisites
Department BIOLOGIA MOLECULAR
Coordinador
LETEK POLBERG , MICHAL
E-mail mletp@unileon.es
smarm@unileon.es
Lecturers
LETEK POLBERG , MICHAL
MARTÍNEZ MARTÍNEZ, SONIA
Web http://
General description This subject aims to delve in a practical way into the diagnostic techniques commonly used in medical microbiology.
Tribunales de Revisión
Tribunal titular
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR FERNANDEZ LOPEZ , ARSENIO
Secretario BIOLOGIA MOLECULAR SAENZ DE MIERA CARNICER , LUIS ENRIQUE
Vocal BIOLOGIA MOLECULAR MARTINEZ PASTOR , FELIPE
Tribunal suplente
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR GONZALEZ CORDERO , ANA ISABEL
Secretario BIOLOGIA MOLECULAR GARCIA GARCIA , PEDRO
Vocal BIOLOGIA MOLECULAR POLANCO DE LA PUENTE , CARLOS

Competencies
Type A Code Competences Specific
Type B Code Competences Transversal
  B5753
  B5754
  B5755
  B5756
  B5757
  B5758
  B5759
  B5760
  B5761
  B5762
  B5763
  B5764
  B5765
  B5766
  B5767
  B5768
Type C Code Competences Nuclear
  C1
  C2
  C3
  C4
  C5

Learning aims
Competences
Delve into: Diagnostic tests in medical microbiology: Culturing techniques for microorganisms from clinical samples Microscopy techniques for the identification of pathogenic bacteria Biochemical tests Molecular identification of pathogens (e.g., RT-PCR) Antibiotic susceptibility testing Minimum inhibitory concentration Surface disinfection B5753
B5754
B5755
B5756
B5757
B5758
B5759
B5760
B5761
B5762
B5763
B5764
B5765
B5766
B5767
B5768
C1
C2
C3
C4
C5
Methods for isolating microorganisms Safety standards in the Microbiology Laboratory Management of DNA databases B5753
B5754
B5755
B5764
B5766
B5767
B5768
C1
C2
C3
C4
C5

Contents
Topic Sub-topic
Lectures Biosafety in the medical microbiology laboratory
Cleaning and disinfection techniques for laboratory surfaces
Laboratory practicals Identification of pathogenic bacteria from clinical samples (e.g., blood, urine, sputum, exudates) through their cultivation in selective media,
Performing rapid staining techniques for the identification of pathogenic bacteria,
Standardized biochemical tests
Use of RT-PCR in pathogen identification
Antibiotic susceptibility testing using the Kirby-Bauer method and Epsilon tests.

Planning
Methodologies  ::  Tests
  Class hours Hours outside the classroom Total hours
Laboratory practicals 24 0 24
 
Personal tuition 2 0 2
 
Lecture 4 0 4
 
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies   ::  
  Description
Laboratory practicals Experimental assays, problem-solving, or computer simulations. Students will perform or follow an experimental protocol adhering to good laboratory practices. In these sessions, both students and the instructor will discuss the results obtained, analyzing their significance and interest.
Personal tuition Specialized in-person or online tutorials through educational support platforms.
Lecture Presentation or discussion of the subject matter content.

Personalized attention
 
Personal tuition
Description
Specialized in-person or online tutorials through educational support platforms.

Assessment
  Description Qualification
Lecture Attendance and active participation in all educational activities. 30%
Laboratory practicals Submission of laboratory practice reports. 40%
Others Assessment of daily work in the laboratory (participation, organization, cleanliness, handling of equipment and materials, attentiveness, interest, and accurate data recording). 30%
 
Other comments and second call

All evaluation tests will be governed by the "Regulation for the Evaluation and Grading of Learning at ULE" (Approved by the Governing Council of ULE on 03-12-2010) and by the document "Guidelines for action in cases of plagiarism, copying, or fraud in exams or evaluation tests" (Approved by the Permanent Commission of the Governing Council of ULE on 01/29/2015).


Sources of information
Access to Recommended Bibliography in the Catalog ULE

Basic Bailey & Scott's, Diagnostic Microbiology, Elsevier, 14th Edition
Shetty, Tang and Andrews, Infectious Disease, Wiley-Blackwell, 1st Edition
Murray, Rosenthal and Pfaller, Medical Microbiology, Elsevier, 8th Edition
Robert W. Bauman, Microbiology with Diseases by Taxonomy, Pearson, 4th Edition
Gerard J. Tortora, Microbiology: An Introduction, Pearson, 12th Edition
Richard V. Goering, MIMS' Medical Microbiology and Inmunology, Elsevier, 6th Edition
Connie R. Mahon, Textbook of Diagnostic Microbiology, Elsevier, 4th Edition

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