Educational guide
IDENTIFYING DATA 2023_24
Subject ANIMAL CELL CULTURE Code 01745007
Study programme
1745 - Máster Universitario en Investigación en Biotecnología y Biomedicina
Descriptors Credit. Type Year Period
3 Compulsory First Second
Language
Castellano
Ingles
Prerequisites
Department BIOLOGIA MOLECULAR
Coordinador
MARÍN VIEIRA, MARÍA CARMEN
 E-mail mmarv@unileon.es

mvvils@unileon.es
llopf@unileon.es
nmarg@unileon.es
avazj@unileon.es
Lecturers
FERNÁNDEZ ALONSO , ROSALIA
VILLAR SUÁREZ , MARÍA VEGA
LOPEZ FERRERAS , LORENA
MARÍN VIEIRA, MARÍA CARMEN
MARTINEZ GARCIA , NICOLE
VAZQUEZ JIMENEZ , ALBERTO
Web http://www.unileon.es/estudiantes/estudiantes-master/oferta-titulaciones/mu-metodologia-investigacion-biologia-biomedicina/plan-estudios?id=0212010&curso=2013
General description The aim of this hands-on course is to familiarize the student with the basic Tissue Culture Techniques of mammalian cells. Moreover, in this subject the theory lectures will also cover the of biology of cultured cells and its biotechnological applications.
Tribunales de Revisión
Tribunal titular
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR FERNANDEZ LOPEZ , ARSENIO
Secretario BIOLOGIA MOLECULAR HERRÁEZ ORTEGA , MARÍA PAZ
Vocal BIOLOGIA MOLECULAR POLANCO DE LA PUENTE , CARLOS
Tribunal suplente
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR PAZ CABELLO , PAULINO DE
Secretario BIOLOGIA MOLECULAR MARTINEZ PASTOR , FELIPE
Vocal CIENCIAS BIOMEDICAS MAURIZ GUTIERREZ , JOSE LUIS

Competencies
Type A Code Competences Specific
  A18956
  A18957
  A18958
Type B Code Competences Transversal
  B5754
  B5757
  B5762
  B5764
  B5766
  B5767
  B5768
Type C Code Competences Nuclear
  C1
  C2
  C3

Learning aims
Competences
- To deepen in the methodological and practical bases of animal cell cultures. A18956
A18957
A18958
 B5754
B5762
B5768
 C2
- To understand the molecular aspects of the biology of animal cells in culture and to apply this knowledge to the design and implementation of cell cultures. A18958
 B5766
B5767
B5768
 C1
-To understand the growth parameters that determine the cell culture and to analyze them by means of growth curves. B5754
B5757
B5764
B5768
 C3

Contents
Topic Sub-topic
Animal cell culture techniques .Basic cell culture techniques: media preparation, sub-culturing, freezing/thawing and detection of cell contamination.
2. Analysis of culture growth kinetics.
3. Establishment and culture of primary cell lines.
Use of animal cell cultures 4. Immortalization and cellular transformation.
5. In vitro models of cell differentiation.

Planning
Methodologies  ::  Tests
  Class hours Hours outside the classroom Total hours
Laboratory practicals 55 0 55
 
Tutorship of group 5 0 5
 
Lecture 15 0 15
 
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies   ::  
  Description
Laboratory practicals Practical classroom sessions: Throughout the course, students will perform experimental activities (practices) in the corresponding laboratories under supervision. The professors will provide the bibliographic documentation, scripts, etc. required in each case. All this material will be available to the students in the institutional platform. The students will be responsible for their experiments, from the preparation of media and solutions, the performance of the experiments, data collection and discussion of the results, always under the supervision of the professor. The students must present a final report including all the experiment performed, written as a scientific work, which will be used by the professors to evaluate their work. Students will perform the experimental procedures accordint to the given protocols and following the guide of good practices for handling experimental animals and/or genetically manipulated microorganisms. The results obtained in each session will be discussed by the students and the professor, analyzing their importance and interest. Self-teaching activities: Analysis of relevant scientific articles on current topics related to the subject. Preparation of written reports, oral presentations or seminars on experimental or bibliographical work. Autonomous tasks online or in a computer classroom.
Tutorship of group Group tutorials Tutorials could be individual or have a group nature to be arranged between the teacher and the students.
Lecture Presentation and discussion of the contents of the subjects. Introduction of the theoretical concepts necessary for the understanding and realization of the practices.

Personalized attention
 
Lecture
Laboratory practicals
Description
Specialized face-to-face and/or online tutoring through educational support platforms.

Assessment
  Description Qualification
Lecture The reports presented will be graded. 20
Laboratory practicals Laboratory practices Attendance to theorical sessions and laboratory practices will be graded in all training activities (attendance and active participation will be considered).
Students will be evaluated during the practical sessions according to their daily work.

 80
 
Other comments and second call

Students will be evaluated during the practical sessions on the basis of their daily work and the laboratory reports submitted. In addition, specific controls of some aspects of the course may be carried out. The grading system will be in accordance with the stipulations of RD 1125/2003.


Sources of information
Access to Recommended Bibliography in the Catalog ULE

Basic

Freshney RI, Culture of Animal Cells. A Manual of Basic Technique, Eighth Edition

Other sources of information will be uploaded to the Moodle platform.

Complementary

All the necessary documents for the follow-up of the course will be uploaded in the institutional Moodle by the professors. 


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