Educational guide
IDENTIFYING DATA 2023_24
Subject ORGAN TISSUE ASSAYS Code 01745008
Study programme
1745 - Máster Universitario en Investigación en Biotecnología y Biomedicina
Descriptors Credit. Type Year Period
3 Compulsory First Second
Language
Castellano
Ingles
Prerequisites
Department BIOLOGIA MOLECULAR
Coordinador
LOPEZ FERRERAS , LORENA
E-mail llopf@unileon.es
aferl@unileon.es
lmaea@unileon.es
Lecturers
FERNÁNDEZ LÓPEZ , ARSENIO
LOPEZ FERRERAS , LORENA
MAESO ALONSO , LAURA
Web http://
General description The isolated organ technique is a useful tool to evaluate the pharmacological activity of a molecule in tissue receptors or channels. In this course, we will work on the basic concepts necessary to carry out a research project based on functional studies using the brain as an isolated organ model. In addition, the intention is to integrate the used methodology with biochemical and physiological concepts to allow for the student´s maximum understanding.
Tribunales de Revisión
Tribunal titular
Cargo Departamento Profesor
Presidente CIENCIAS BIOMEDICAS SANCHEZ CAMPOS , SONIA
Secretario MEDICINA,CIRUGIA Y ANAT.VETERI PEREZ GARCIA , CARLOS CESAR
Vocal CIENCIAS BIOMEDICAS TUÑON GONZALEZ , MARIA JESUS
Tribunal suplente
Cargo Departamento Profesor
Presidente PRODUCCION ANIMAL MARQUES MARTINEZ , MARIA MARGARITA
Secretario MEDICINA,CIRUGIA Y ANAT.VETERI DIEZ PRIETO , INMACULADA
Vocal BIOLOGIA MOLECULAR MARTINEZ PASTOR , FELIPE

Competencies
Type A Code Competences Specific
Type B Code Competences Transversal
  B5753
  B5754
  B5755
  B5756
  B5757
  B5758
  B5759
  B5760
  B5761
  B5762
  B5763
  B5764
  B5765
  B5766
  B5767
  B5768
Type C Code Competences Nuclear
  C1
  C2
  C3
  C4

Learning aims
Competences
The student must be able to prepare specific media from given recipes. B5766
Theoretical-practical knowledge of the methodological foundations of isolated organ experimentation and its application to research and development and/or other professional activities. B5760
B5766
B5768
Know the resources to search for information, have reliability criteria for sources and collect and interpret data. B5766
B5767
 C4
Lay the foundations for the general planning aspects of experimental work, with a rational use of media and resources, as well as the establishment of useful criteria for data collection and analysis. B5753
B5757
B5762
B5763
 C1
C2
Solve problems that may arise in the laboratory or at least know what to do. B5754
B5758
Write and properly present the results of an essay. B5753
B5756
Use Internet resources both to search for information and to use forums or other ways of communication. B5755
Be able to communicate the results obtained in a precise and effective way. B5753
B5756
 C3
Be able to plan essays in a structured and effective way. B5761
B5767
B5768
Make decisions about the design of an experiment or the solution of a problem. B5754
B5758
B5763
Ability to distribute tasks among team members. B5757
B5759
B5762
Capacity for self-criticism and to learning from mistakes made during the experimental procedures. B5764
 C2
Design alternatives from the usual protocols. B5754
B5763
B5764
B5767
 C1
Work effectively as a team, promoting discussion and discussing different points of view in a conciliatory way. B5757
B5759
B5762
Critical capacity to evaluate information in a weighted way. B5764
Find alternatives when existing resources are not enough to solve a problem. B5754
B5763
Maintain ethical criteria B5765

Contents
Topic Sub-topic
Methodology for the isolated organ technique Preparation of media for isolated organ essays.
Isolation of brain sections as an "ex vivo" essay model
Practical essay • Dose-response curves.
• Mortality and viability assays.

Planning
Methodologies  ::  Tests
  Class hours Hours outside the classroom Total hours
Laboratory practicals 25 38 63
 
Personal tuition 1 1 2
 
Lecture 3 3 6
 
Objective multiple-choice tests 2 2 4
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies   ::  
  Description
Laboratory practicals Students will follow an experimental protocol following good laboratory practices and the Good Practice Guide for the use of animals in research, testing and teaching. In these sessions, the results obtained will be discussed by the students and the teacher, analyzing their importance and interest.
Personal tuition Face-to-face or online personal meetings through educational support platforms
Lecture Master class with discussion of the contents of the course

Personalized attention
 
Personal tuition
Description
Face-to-face or online personal meetings through educational support platforms

Assessment
  Description Qualification
Laboratory practicals Continuous assessment of daily work in the laboratory (participation, order, cleanliness, handling of equipment, material, samples and animals, attention, interest and annotated record of data...) 60
Lecture Multiple choice tests. The rating system will be adjusted to the one stipulated in RD 1125/2003 40
 
Other comments and second call

Sources of information
Access to Recommended Bibliography in the Catalog ULE

Basic

Students must search for information using the web page http://www.ncbi.nlm.nih.gov/pubmed/. The bibliographical sources will be reviewed by the teacher.

Complementary


Recommendations