Educational guide
IDENTIFYING DATA 2023_24
Subject ANIMAL CELL CULTURE APPLICATIONS Code 01745010
Study programme
1745 - Máster Universitario en Investigación en Biotecnología y Biomedicina
Descriptors Credit. Type Year Period
3 Optional First Second
Language
Castellano
Ingles
Prerequisites
Department MEDICINA,CIRUGIA Y ANAT.VETERI
Coordinador
VILLAR SUÁREZ , MARÍA VEGA
E-mail mvvils@unileon.es
mletp@unileon.es
mmarm@unileon.es
mmarv@unileon.es
Lecturers
LETEK POLBERG , MICHAL
MARQUÉS MARTÍNEZ , MARGARITA
VILLAR SUÁREZ , MARÍA VEGA
MARÍN VIEIRA, MARÍA CARMEN
Web http://
General description This subject aims to delve in a practical way with the basic techniques of cell culture and their biotechnological and health-related applications.
Tribunales de Revisión
Tribunal titular
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR FERNANDEZ LOPEZ , ARSENIO
Secretario CIENCIAS BIOMEDICAS CUEVAS GONZALEZ , MARIA JOSE
Vocal PRODUCCION ANIMAL GUTIERREZ GIL , BEATRIZ
Tribunal suplente
Cargo Departamento Profesor
Presidente BIOLOGIA MOLECULAR FERNANDEZ CAÑON , JOSE MANUEL
Secretario PRODUCCION ANIMAL SANCHEZ SANCHEZ , JOSE MARIA
Vocal CIENCIAS BIOMEDICAS SANCHEZ CAMPOS , SONIA

Competencies
Type A Code Competences Specific
  A18948
  A18953
  A18956
  A18957
  A18958
Type B Code Competences Transversal
  B5753
  B5754
  B5755
  B5757
  B5758
  B5760
  B5762
  B5763
  B5764
  B5765
  B5766
  B5767
  B5768
Type C Code Competences Nuclear
  C1
  C2
  C4
  C5

Learning aims
Competences
A18948
 B5753
B5754
B5755
B5757
B5764
B5765
B5766
B5767
B5768
 C1
C2
C4
C5
A18948
A18958
 B5755
B5757
B5764
 C1
C2
C4
C5
A18948
A18953
A18956
A18957
A18958
 B5753
B5754
B5755
B5757
B5758
B5760
B5762
B5763
B5764
B5765
B5766
B5767
B5768
 C1
C2
C4
C5

Contents
Topic Sub-topic
PRIMARY CELL CULTURES • Establishment of primary cell cultures for use in biomedical applications.
• Cellular models of differentiation.
GENETIC MODIFICATION • Animal cell transfection and transduction methods. Generation of stable cell lines that express genes of interest.
IN VITRO INFECTION MODELS • In vitro infection tests of biomedical interest.

Planning
Methodologies  ::  Tests
  Class hours Hours outside the classroom Total hours
Laboratory practicals 20 30 50
 
Personal tuition 1 1.5 2.5
Portfolios/Learning folder 1 1.5 2.5
 
Lecture 8 12 20
 
 
(*)The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies   ::  
  Description
Laboratory practicals In-person practical sessions. Experimental tests, problems or computer simulations. Students will carry out or follow an experimental protocol following good laboratory practices, the guide to good practices for handling experimental animals and/or genetically manipulated microorganisms. In these sessions, the results obtained will be discussed by the students and the teacher, analysing their importance and interest.
Personal tuition Specialized face-to-face online tutoring through educational support platforms.
Portfolios/Learning folder Self-training activities: -Analysis of relevant scientific articles on current topics related to the subject. Preparation of written reports, oral presentations or seminars on experimental or bibliographic work. - Carrying out autonomous tasks online or in a computer room.
Lecture Face-to-face theoretical sessions. Presentation or discussion of the contents of the subject.

Personalized attention
 
Description

Assessment
  Description Qualification
Others Attendance at laboratory practices (attendance and active participation in all training activities).

Delivery of practice report.		 60



40%
 
Other comments and second call

Sources of information
Access to Recommended Bibliography in the Catalog ULE

Basic

Freshney RI, Culture of Animal Cells. A Manual of BasicTechnique, Fourth Edition

Ranjna C. Dutta, Aroop K. Dutta. 3D Cell Culture: Fundamentals and Applications in Tissue Engineering and Regenerative Medicine.

Cell Culture and Its Application. Ronald T. Acton, J. Daniel Lynn.

Complementary

All the material necessary to follow the subject will be posted on Moodle.


Recommendations


Subjects that it is recommended to have taken before
ANIMAL CELL CULTURE / 01745007